Some properties of beta-toxin produced by Clostridium perfringens type C

Some properties of Beta Toxin produced by Clostridium haemolyticum strain IRP 13

Purification of beta-toxin from Clostridium perfringens type C.

Beta-toxin was purified about 340-fold from culture supernatant fluid of Clostridium perfringens type C with a yield of about 24% in terms of biologically active beta-toxin. The purification involved ammonium sulfate fractionation, gel filtration through Sephadex G-100, isoelectrofocusing in a pH 3 to 6 gradient, and immunoaffinity chromatography. The purified beta-toxin gave a single band on p...

Molecular Cloning of Clostridium Perfringens type B Vaccine Strain Beta Toxin Gene in E. coli

Clostridium perfringens is a gram-positive, obligate anaerobic bacterium, which is widely distributed in the environment. C. perfringens is subdivided to 5 groups (types A to E), based on its four major toxin (alpha, beta, epsilon and iota). C. perfringens type B beta toxin causes inflammation and bloody necrotic enteritis. Type B related enterotoxaemia is a major problem of veterinary sciences...

a new purification method for beta-toxin of clostridium perfringens type c vaccinal strain

background : clostridium perfringens type c strains cause severe necrotizing enteritis in sheep, calves, goats, pigs and humans. beta-toxin is introduced to be the essential virulence factor of this microorganism. in the present study, a new method was established for the purification of beta toxin from culture supernatant fluid of c. perfringens type c vaccinal strain. methods : the four steps...

some properties of beta toxin produced by clostridium haemolyticum strain irp 13

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